Apoptosis Methods and Protocols

Apoptosis Methods and Protocols (Methods in Molecular Biology Vol 282)
425 pages | Aug 31 2010 |ISBN:0896038734 | PDF | 3.5 Mb

A collection of cutting-edge techniques for detecting and quantifying apoptosis, understanding its biochemistry, and for identifying the genes and proteins that regulate and carry it out. Described in step-by-step detail, these readily reproducible methods range from flow cytometry and immunohistochemical procedures to kinase activity assays, yeast two-hybrid screening, and the cloning of novel genes by differential expression. The protocols follow the successful Methods in Molecular Biology series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique

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